Heather D. Agnew
Work Phone Number:
6162 Bristol Parkway
Culver City, CA 90230
Department / Division Affiliations
|Member, Molecular Pharmacology GPB Home Area|
|Adjunct Assistant Professor, Molecular & Medical Pharmacology|
Most clinical technologies for detecting protein biomarkers are antibody based. As an alternative approach, my research focuses on the development of protein-catalyzed capture agents (PCCs) compounds that possess the affinities and specificities of antibodies, but also are highly stable on the shelf and in vivo. PCCs are selected for specific binding through synthetic combinatorial peptide library methodologies that make use of the target protein as the catalyst for assembling a multivalent ligand. We are exploiting the small size, low cost, and rapid synthesis of PCCs to monitor biomarkers to detect cancer and other serious diseases.
M. B. Coppock, C. R. Warner, B. Dorsey, J. A. Orlicki, D. A. Sarkes, B. T. Lai, S. M. Pitram, R. D. Rohde, J. Malette, J. A. Wilson, P. Kearney, K. C. Fang, S. M. Law, S. L. Candelario, B. Farrow, A. S. Finch, H. D. Agnew, J. R. Heath, and D. N. Stratis-Cullum. Protein Catalyzed Capture Agents with Tailored Performance for In Vitro and In Vivo Applications. Biopolymers (Pept Sci). 2017; 108(2): e22934.
S. Das, A. Nag, J. Liang, D. N. Bunck, A. Umeda, B. Farrow, M. B. Coppock, D. A. Sarkes, A. S. Finch, H. D. Agnew, S. Pitram, B. Lai, M. B. Yu, A. K. Museth, K. M. Deyle, B. Lepe, F. P. Rodriguez-Rivera, A. McCarthy, B. Alvarez-Villalonga, A. Chen, J. Heath, D. N. Stratis-Cullum, and J. R. Heath. A General Synthetic Approach for Designing Epitope Targeted Macrocyclic Peptide Ligands. Angew Chem Int Ed. 2015; 54(45): 13219-13224.
B. Farrow, M. Wong, J. Malette, B. Lai, K. M. Deyle, S. Das, A. Nag, H. D. Agnew, and J. R. Heath. Epitope Targeting of Tertiary Protein Structure Enables Target-Guided Synthesis of a Potent In-Cell Inhibitor of Botulinum Neurotoxin. Angew Chem Int Ed. 2015; 54(24): 71147119.
B. Farrow, S. A. Hong, E. Romero, B. Lai, M. B. Coppock, K. M. Deyle, A. S. Finch, D. N. Stratis-Cullum, H. Agnew, S. Yang, and J. R. Heath. A Chemically Synthesized Capture Agent Enables the Selective, Sensitive, and Robust Electrochemical Detection of Anthrax Protective Antigen. ACS Nano. 2013; 7(10): 94529460.
S. W. Millward, H. D. Agnew, B. Lai, S. S. Lee, J. Lim, A. Nag, S. Pitram, R. Rohde, and J. R. Heath. In Situ Click Chemistry: From Small Molecule Discovery to Synthetic Antibodies. Integr Biol. 2013; 5(1): 87-95.
S. W. Millward, H. D. Agnew, S. Pitram, B. T. Lai, R. D. Rohde, and N. Hardman. Protein Catalysed Capture Agents for Molecular Imaging. International Hospital Equipment & Solutions. 2013; (March/April): 14-16.
C. H. Sohn, H. D. Agnew, J. E. Lee, M. J. Sweredoski, R. L. J. Graham, G. T. Smith, S. Hess, G. Czerwieniec, J. A. Loo, J. R. Heath, R. J. Deshaies, and J. L. Beauchamp. Designer Reagents for Mass Spectrometry-Based Proteomics: Clickable Cross-Linkers for Elucidation of Protein Structures and Interactions. Anal Chem. 2012; 84(6): 2662-2669.
S. W. Millward, R. K. Henning, G. A. Kwong, S. Pitram, H. D. Agnew, K. M. Deyle, A. Nag, J. Hein, S. S. Lee, J. Lim, K. B. Sharpless, and J. R. Heath. Iterative In Situ Click Chemistry Assembles a Branched Capture Agent and Allosteric Inhibitor for Akt1. J Am Chem Soc. 2011; 133(45): 18280-18288.
S. S. Lee, J. Lim, S. Tan, J. Cha, S. Y. Yeo, H. D. Agnew, and J. R. Heath. Accurate MALDI-TOF/TOF Sequencing of One-Bead-One-Compound Peptide Libraries with Application to the Identification of Multiligand Protein Affinity Agents Using in situ Click Chemistry Screening. Anal Chem. 2010; 82(2): 672-679.
H. D. Agnew, R. D. Rohde, S. W. Millward, A. Nag, W.-S. Yeo, J. E. Hein, S. M. Pitram, A. A. Tariq, V. M. Burns, R. J. Krom, V. V. Fokin, K. B. Sharpless, and J. R. Heath. Iterative in situ Click Chemistry Creates Antibody-like Protein Capture Agents. Angew Chem Int Ed. 2009; 48(27): 4944-4948.
M. C. McAlpine, H. D. Agnew, R. D. Rohde, M. Blanco, H. Ahmad, A. D. Stuparu, W. A. Goddard III, and J. R. Heath. Peptide-Nanowire Hybrid Materials for Selective Sensing of Small Molecules. J Am Chem Soc. 2008; 130(29): 9583-9589.
O. S. Miljanic, W. R. Dichtel, I. Aprahamian, R. D. Rohde, H. D. Agnew, J. R. Heath, and J. F. Stoddart. Rotaxanes and Catenanes by Click Chemistry. QSAR Comb Sci. 2007; 26(11-12): 1165-1174.
R. D. Rohde, H. D. Agnew, W.-S. Yeo, R. C. Bailey, and J. R. Heath. A Non-Oxidative Approach toward Chemically and Electrochemically Functionalizing Si(111). J Am Chem Soc. 2006; 128(29): 9518-9525.
J. J. Lichty, J. L. Malecki, H. D. Agnew, D. J. Michelson-Horowitz, and S. Tan. Comparison of Affinity Tags for Protein Purification. Prot Expr Purif. 2005; 41(1): 98-105.