FACULTY RESEARCH

Faculty List

Jing Huang, Ph.D.

Work Email Address: jinghuang@mednet.ucla.edu

Lab Number: (310) 206-3619

Office Phone Number: (310) 825-4329

Jing Huang Lab

Jing Huang Lab

Laboratory Address:
23-231 CHS
650 Charles E. Young Drive South
Los Angeles, CA 90095

Office Address:
23-231A CHS
Department of Molecular & Medical Pharmacology

Department / Division Affiliations
Professor, Molecular & Medical Pharmacology
Member, JCCC Signal Transduction and Therapeutics Program Area

Research Interests
1. Longevity molecules that counteract aging and extend lifespan have long been a dream of humanity. Compared to ad libitum feeding, dietary restriction (DR) consistently extends lifespan and prevents age-related diseases. We discovered that a common metabolite, alpha-ketoglutarate (a-KG), acts as a direct molecular connection between dietary restriction and major aging regulatory proteins (Chin et al. 2014). Endogenous molecules such as a-KG that increase longevity suggest that an internal "fountain of youth" may exist that is accessible to interventions. Future research in this area offers enormous hopes and possibilities for better understanding of the molecular mechanisms of aging regulation, and for the prevention and treatment of age-related diseases, including major common killers such as cancer, heart disease, diabetes, and neurodegenerative diseases. 2. A second major area of interest in the lab is in technology and method development. Development of effective and safe therapies is the holy grail of medicine. For small-molecule drugs, a key challenge remains the identification of the molecular targets underlying drug therapeutic effects and/or adverse side effects. We have developed a simple, universally applicable target identification approach that analyzes direct drug binding to its target protein but does not require modification or immobilization of the small molecule (Lomenick et al. 2009). In this method, termed DARTS (drug affinity responsive target stability), small-molecule binding results in a pronounced and specific protection of its target protein from protease digestion. This specificity can be exploited to identify unknown binding targets of a small molecule with any cell lysate or protein mixture. labs.pharmacology.ucla.edu/huanglab/


Publications
Xudong Fu, Randall M. Chin, Laurent Vergnes, Heejun Hwang, Gang Deng, Yanpeng Xing, Melody Y. Pai, Sichen Li, Lisa Ta, Farbod Fazlollahi, Chuo Chen, Robert M. Prins, Michael A. Teitell, David A. Nathanson, Albert Lai, Kym F. Faull, Meisheng Jiang, Steven G. Clarke, Timothy F. Cloughesy, Thomas G. Graeber, Daniel Braas, Heather R. Christofk, Michael E. Jung, Karen Reue, Jing Huang 2-Hydroxyglutarate inhibits ATP synthase and mTOR signaling. Cell Metabolism. 2015; 22: 508-515.
Chin Randall M, Fu Xudong, Pai Melody Y, Vergnes Laurent, Hwang Heejun, Deng Gang, Diep Simon, Lomenick Brett, Meli Vijaykumar S, Monsalve Gabriela C, Hu Eileen, Whelan Stephen A, Wang Jennifer X, Jung Gwanghyun, Solis Gregory M, Fazlollahi Farbod, Kaweeteerawat Chitrada, Quach Austin, Nili Mahta, Krall Abby S, Godwin Hilary A, Chang Helena R, Faull Kym F, Guo Feng, Jiang Meisheng, Trauger Sunia A, Saghatelian Alan, Braas Daniel, Christofk Heather R, Clarke Catherine F, Teitell Michael A, Petrascheck Michael, Reue Karen, Jung Michael E, Frand Alison R, Huang Jing The metabolite α-ketoglutarate extends lifespan by inhibiting ATP synthase and TOR. Nature. 2014; 510(7505): 397-401.
Jing Huang Tracking Drugs. The New England Journal of Medicine. 2013; 369: 1168-1169.
Tyler JW Robinson, Melody Pai, Jeff C Liu, Frederick Vizeacoumar, Thomas Sun, Sean E Egan, Alessandro Datti, Jing Huang, Eldad Zacksenhaus High-throughput screen identifies disulfiram as a potential therapeutic for triple-negative breast cancer cells: Interaction with IQ motif-containing factors. Cell Cycle. 2013; 12(18): 3013-3024.
Gao, S. Wang, Q. Wang, G. Lomenick, B. Liu, J. Fan, C. W. Deng, L. W. Huang, J. Lum, L. Chen, C. The Chemistry and Biology of Nakiterpiosin - C-nor-D-Homosteroids. Synlett. 2012; 16(23): 2298-2310.
Lomenick, B. Jung, G. Wohlschlegel, J. A. Huang, J. Target identification using drug affinity responsive target stability (DARTS). Curr Protoc Chem Biol. 2011; 3(4): 163-180.
Visnyei, K. Onodera, H. Damoiseaux, R. Saigusa, K. Petrosyan, S. De Vries, D. Ferrari, D. Saxe, J. Panosyan, E. H. Masterman-Smith, M. Mottahedeh, J. Bradley, K. A. Huang, J. Sabatti, C. Nakano, I. Kornblum, H. I. A molecular screening approach to identify and characterize inhibitors of glioblastoma stem cells. Mol Cancer Ther. 2011; 10(10): 1818-1828.
Watanabe, R. Wei, L. Huang, J. mTOR signaling, function, novel inhibitors, and therapeutic targets. J Nucl Med. 2011; 52(4): 497-500.
Lomenick, B. Olsen, R. W. Huang, J. Identification of direct protein targets of small molecules. ACS Chem Biol. 2011; 6(1): 34-46.
Mariam Aghajan, Nao Jonai, Karin Flick, Fei Fu, Manlin Luo, Xiaolu Cai, Ikram Ouni, Nathan Pierce, Xiaobo Tang, Brett Lomenick, Robert Damoiseaux, Rui Hao, Pierre M del Moral, Rati Verma, Ying Li, Cheng Li, Kendall N Houk, Michael E Jung, Ning Zheng, Lan Huang, Raymond J Deshaies, Peter Kaiser & Jing Huang Chemical genetics screen for enhancers of rapamycin identifies a specific inhibitor of an SCF family E3 ubiquitin ligase. Nature Biotechnology. 2010; doi:10.1038/nbt.1645 published online 27 June 2010: .
Brett Lomenick, Rui Hao, Nao Jonai, Randall M. Chin, Mariam Aghajan, Sarah Warburton, Jianing Wang, Raymond P. Wu, Fernando Gomez, Joseph A. Loo, James A. Wohlschlegel, Thomas M. Vondriska, Jerry Pelletier, Harvey R. Herschman, Jon Clardy, Catherine F. Clarke, and Jing Huang Target identification using drug affinity responsive target stability (DARTS). Proc Natl Acad Sci USA. 2009; 106(51): 21984-21989.
Jonathan P. Saxe, Alexey Tomilin, Hans R. Schöler, Kathrin Plath, and Jing Huang Post-translational regulation of Oct4 transcriptional activity. PLoS ONE. 2009; 4(2): e4467.
Lihao Meng, Gregory A Michaud, Janie S Merkel, Fang Zhou, Jing Huang, Dawn R Mattoon and Barry Schweitzer Protein kinase substrate identification on functional protein arrays. BMC Biotechnol. 2008; 8(1): 22.
Heng Zhu and Jing Huang A combined force of chemical genetics and protein microarrays. Functional Protein Microarrays in Drug Discovery, Paul F. Predki, ed. 2007; .
Jonathan P. Saxe, Hao Wu, Theresa K. Kelly, Michael E. Phelps, Yi E. Sun, Harley I. Kornblum, and Jing Huang A phenotypic small-molecule screen identifies an orphan ligand-receptor pair that regulates neural stem cell differentiation. Chemistry & Biology. 2007; 14(9): 1019-30.
Download
Fulai Jin, Larisa Avramova, Jing Huang & Tony Hazbun A yeast two-hybrid smart-pool-array system for protein-interaction mapping. Nature Methods. 2007; 4(5): 405-7.
Download
Mara C. Duncan, David G. Ho, Jing Huang, Michael E. Jung, Gregory S. Payne Composite synthetic lethal identification of membrane traffic inhibitors. Proc Natl Acad Sci USA. 2007; 104(15): 6235-40.
Download
Jin F, Hazbun T, Michaud GA, Salcius M, Predki PF, Fields S & Huang J A pooling-deconvolution strategy for biological network elucidation. Nature Methods. 2006; 3(3): 183-9.
Download
Masakazu Kamata, Raymond P. Wu, Dong Sung An, Jonathan P. Saxe, Robert Damoiseaux, Michael E. Phelps, Jing Huang, Irvin S.Y. Chen Cell-based chemical genetic screen identifies damnacanthal as an inhibitor of HIV-1 Vpr induced cell death. Biochem Biophys Res Commun. 2006; 348(3): 1101-6.
Download
Xie MW, Jin F, Hwang H, Hwang S, Anand V, Duncan MC, and Huang J Insights into TOR function and rapamycin response: chemical genomic profiling by using a high-density cell array method. Proc Natl Acad Sci U S A. 2005; 102(20): 7215-20.
Download
Huang J, Zhu H, Haggarty SJ, Spring DR, Hwang H, Jin F, Snyder M and Schreiber SL Finding new components of the target of rapamycin (TOR) signaling network through chemical genetics and proteome chips. Proc Natl Acad Sci U S A. 2004; 101(47): 16594-9.
Download
Paulmurugan R, Massoud TF, Huang J and Gambhir SS Molecular imaging of drug-modulated protein-protein interactions in living subjects. Cancer Research. 2004; 64(6): 2113-9.
Download
Zewail A, Xie MW, Xing Y, Lin L, Zhang PF, Zou W, Saxe JP and Huang J Novel functions of the phosphatidylinositol metabolic pathway discovered by a chemical genomics screen with wortmannin. Proc Natl Acad Sci U S A. 2003; 100(6): 3345-50.
Download
Huang J, Haggarty SJ and Schreiber SL Understanding target-of-rapamycin function through small molecules and microarrays. FASEB J 2000; 14(8): 1410.
Blackwell TK, Huang J, Ma A, Kretzner L, Alt FW, Eisenman RN and Weintraub H Binding of Myc proteins to canonical and non-canonical DNA sequences. Mol Cell Biol. 1993; 13(9): 5216-24.
Huang J, Weintraub H and Kedes L Intramolecular regulation of MyoD activation domain conformation and function. Mol Cell Biol. 1998; 18(9): 5478-84.
Huang J and Schreiber SL A yeast genetic system for selecting small molecule inhibitors of protein-protein interactions in nanodroplets. Proc Natl Acad Sci U S A. 1997; 94(25): 13396-401.
Sartorelli V, Huang J, Hamamori Y and Kedes L Molecular mechanisms of myogenic coactivation by p300: direct interaction with the activation domain of MyoD and with the MADS box of MEF2C. Mol Cell Biol. 1997; 17(2): 1010-26.
Huang J, Blackwell TK, Kedes L and Weintraub H Differences between MyoD DNA binding and activation site requirements revealed by functional random sequence selection. Mol Cell Biol. 1996; 16(7): 3893-900.