FACULTY RESEARCH

Faculty List

Lily Wu, M.D., Ph.D.

Work Email Address: lwu@mednet.ucla.edu

Fax Number: (310) 794-4759
(310) 825-3027

Lab Number: (310) 825-8511

Office Phone Number: (310)794-4390

Annual Research Report Papers

Laboratory Address:
10833 Le Conte Ave.
CHS 33-142
Los Angeles, CA 90095

Work Address:
33-118 CHS
Los Angeles, CA 90095

Department / Division Affiliations
Professor, Molecular & Medical Pharmacology
Associate Professor, Pediatrics, Urology
Member, CTSI, California NanoSystems Institute, JCCC Cancer Molecular Imaging, JCCC Genitourinary Oncology Program Area, Molecular Pharmacology GPB Home Area, Molecular, Cellular & Integrative Physiology GPB Home Area
Investigator, Workforce Development

Research Interests
Our research is aimed at developing a better understanding of cancer biology and creating an improved gene-based therapy by exploiting special properties of the adenovirus. We have utilized the efficient gene transfer property of adenovirus to create improved, tissuetargeted therapeutic strategies for cancer. By applying this targeted gene-expression approach to molecular imaging, metastatic lesions were detected in living tumor-bearing mice. Transcriptional targeted approaches are being developed to express the different therapeutic genes and imaging genes in prostate and breast cancer models. In a second strategy, we are exploiting the distributive property of the adenovirus and use it to investigate tumor vasculatures and cancer metastasis. As a third strategy, the viral replication property is usurped to create cancer-specific lysis. Molecular imaging techniques such as optical CCD imaging and positron emission tomography (PET) are fundamental tools of our investigation. Our ultimate goal is to create a potent and effective cancer-targeted gene therapy to diagnose and treat advanced stage and metastatic cancer.


Publications
Pouliot Frédéric, Sato Makoto, Jiang Ziyue Karen, Huyn Steve, Karanikolas Breanne Dw, Wu Lily A molecular imaging system based on both transcriptional and genomic amplification to detect prostate cancer cells in vivo. Molecular therapy : the journal of the American Society of Gene Therapy. 2013; 21(3): 554-60.
Xu Jingying, Escamilla Jemima, Mok Stephen, David John, Priceman Saul, West Brian, Bollag Gideon, McBride William, Wu Lily CSF1R Signaling Blockade Stanches Tumor-Infiltrating Myeloid Cells and Improves the Efficacy of Radiotherapy in Prostate Cancer. Cancer research. 2013; 73(9): 2782-94.
Jiang Ziyue Karen, Koh Sok Boon S, Sato Makoto, Atanasov Ivo C, Johnson Mai, Zhou Z Hong, Deming Timothy J, Wu Lily Engineering polypeptide coatings to augment gene transduction and in vivo stability of adenoviruses. Journal of controlled release : official journal of the Controlled Release Society. 2013; 166(1): 75-85.
Jiang Ziyue Karen, Sato Makoto, Wu Lily Chapter five--The development of transcription-regulated adenoviral vectors with high cancer-selective imaging capabilities. Advances in cancer research. 2012; 115(15): 115-46.
He Huanhuan, Xu Jingying, Warren Carmen M, Duan Dan, Li Xinmin, Wu Lily, Iruela-Arispe M Luisa Endothelial cells provide an instructive niche for the differentiation and functional polarization of M2-like macrophages. Blood. 2012; 120(15): 3152-62.
Wu Lily, Grigoryan Arsen V, Li Yunfeng, Hao Bing, Pagano Michele, Cardozo Timothy J Specific small molecule inhibitors of Skp2-mediated p27 degradation. Chemistry & biology. 2012; 19(12): 1515-24.
Chen Kuan-Ju, Wolahan Stephanie M, Wang Hao, Hsu Chao-Hsiung, Chang Hsing-Wei, Durazo Armando, Hwang Lian-Pin, Garcia Mitch A, Jiang Ziyue K, Wu Lily, Lin Yung-Ya, Tseng Hsian-Rong A small MRI contrast agent library of gadolinium(III)-encapsulated supramolecular nanoparticles for improved relaxivity and sensitivity. Biomaterials. 2011; 32(8): 2160-5.
Jiang Ziyue Karen, Sato Makoto, Wei Liu H, Kao Chinghai, Wu Lily Androgen-independent molecular imaging vectors to detect castration-resistant and metastatic prostate cancer. Cancer research. 2011; 71(19): 6250-60.
Liu Hongrong, Wu Lily, Zhou Z Hong Model of the trimeric fiber and its interactions with the pentameric penton base of human adenovirus by cryo-electron microscopy. Journal of molecular biology. 2011; 406(5): 764-74.
Narasimha Ajay J, Watanabe Junji, Ishikawa Tomo-o, Priceman Saul J, Wu Lily, Herschman Harvey R, Reddy Srinivasa T Absence of myeloid COX-2 attenuates acute inflammation but does not influence development of atherosclerosis in apolipoprotein E null mice. Arteriosclerosis, thrombosis, and vascular biology. 2010; 30(2): 260-8.
Hongrong Liu, Lei Jin, Sok Boon S. Koh, Ivo Atanasov, Stan Schein, Lily Wu, Z. Hong Zhou Atomic Structure of Human Adenovirus by Cryo-EM Reveals Interactions Among Protein Networks. Science. 2010; 329(5995): 1038-1043.
Liu Hongrong, Jin Lei, Koh Sok Boon S, Atanasov Ivo, Schein Stan, Wu Lily, Zhou Z Hong Atomic structure of human adenovirus by cryo-EM reveals interactions among protein networks. Science (New York, N.Y.). 2010; 329(5995): 1038-43.
Karanikolas Breanne D W, Figueiredo Marxa L, Wu Lily Comprehensive evaluation of the role of EZH2 in the growth, invasion, and aggression of a panel of prostate cancer cell lines. The Prostate. 2010; 70(6): 675-88.
Imaizumi Satoshi, Grijalva Victor, Priceman Saul, Wu Lily, Su Feng, Farias-Eisner Robin, Hama Susan, Navab Mohamad, Fogelman Alan M, Reddy Srinivasa T Mitogen-activated protein kinase phosphatase-1 deficiency decreases atherosclerosis in apolipoprotein E null mice by reducing monocyte chemoattractant protein-1 levels. Molecular genetics and metabolism. 2010; 101(1): 66-75.
Priceman SJ, Sung JL, Shaposhnik Z, Burton JB, Torres-Collado T, Moughon D, Johnson M, Cohen DA, Lusis AJ, Iruela-Arispe L, Wu L Targeting distinct tumor-infiltrating myeloid cells by inhibiting CSF-1 receptor: combating tumor evasion of anti-angiogenic therapy. Blood . 2010; 115(7): 1461-71.
Priceman Saul J, Sung James L, Shaposhnik Zory, Burton Jeremy B, Torres-Collado Antoni X, Moughon Diana L, Johnson Mai, Lusis Aldons J, Cohen Donald A, Iruela-Arispe M Luisa, Wu Lily Targeting distinct tumor-infiltrating myeloid cells by inhibiting CSF-1 receptor: combating tumor evasion of antiangiogenic therapy. Blood. 2010; 115(7): 1461-71.
Ming Yan, Juanjuan Du, Zhen Gu, Min Liang, Yufang Hu, Wenjun Zhang, Saul Priceman, Lily Wu, Z. Hong Zhou, Zheng Liu, Tatiana Segura, Yi Tang, Yunfeng Lu A novel intracellular protein delivery platform based on single-protein nanocapsules. Nature Nanotechnology. 2009; 5: 48-53.
Huyn Steven T, Burton Jeremy B, Sato Makoto, Carey Michael, Gambhir Sanjiv S, Wu Lily A potent, imaging adenoviral vector driven by the cancer-selective mucin-1 promoter that targets breast cancer metastasis. Clinical cancer research : an official journal of the American Association for Cancer Research. 2009; 15(9): 3126-34.
Huyn, S. T. Burton, J. B. Sato, M. Carey, M. Gambhir, S. S. Wu, L. A potent, imaging adenoviral vector driven by the cancer-selective mucin-1 promoter that targets breast cancer metastasis. Clin Cancer Res. 2009; 15(9): 3126-34.
Pouliot F, Johnson M, Wu L Non-invasive molecular imaging of prostate cancer lymph node metastasis. Trends in Molecular Medicine 2009; 15: 254-262.
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Karanikolas Breanne D W, Figueiredo Marxa L, Wu Lily Polycomb group protein enhancer of zeste 2 is an oncogene that promotes the neoplastic transformation of a benign prostatic epithelial cell line. Molecular cancer research : MCR. 2009; 7(9): 1456-65.
Karanikolas BDW, Figueiredo ML, Wu L Polycomb group protein enhancer of zeste 2 is an oncogene that promotes the neoplastic transformation of a benign prostatic epithelial cell line. . Molecular Cancer Research 2009; 7(9): 1456-1465.
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Johnson Mai, Karanikolas Breanne D W, Priceman Saul J, Powell Russell, Black Margaret E, Wu Hsiao-Ming, Czernin Johannes, Huang Sung-Cheng, Wu Lily Titration of variant HSV1-tk gene expression to determine the sensitivity of 18F-FHBG PET imaging in a prostate tumor. Journal of nuclear medicine : official publication, Society of Nuclear Medicine. 2009; 50(5): 757-64.
Johnson M, Karanikolas BDW, Priceman SJ, Powell R, Black ME, Wu HM, Czernin J, Huang SC, Wu L Titration of variant HSV1-tk gene expression to determine the sensitivity of 18F-FHBG PET imaging in a prostate tumor. Journal of Nuclear Medicine 2009; 50: 757-764.
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Burton, J. B. Johnson, M. Sato, M. Koh, S. B. S. Mulholland, D. J. Stout, D. Chatziioannou, A. F. Phelps, M. E. Wu, H. Wu, L. Adenovirus-mediated gene expression imaging to directly detect sentinel lymph node metastasis of prostate cancer. Nature Medicine. 2008; 14(8): 882-888.
Burton Jeremy B, Johnson Mai, Sato Makoto, Koh Sok Boon S, Mulholland David J, Stout David, Chatziioannou Arion F, Phelps Michael E, Wu Hong, Wu Lily Adenovirus-mediated gene expression imaging to directly detect sentinel lymph node metastasis of prostate cancer. Nature medicine. 2008; 14(8): 882-8.
Haven Thomas R, Lyons Bradley W, Pasi Brian M, Wu Lily L, Astill Mark E, Hill Harry R Effect of acetylcholine receptor source on detection and quantification of anti-acetylcholine receptor-binding antibody. Clinical chemistry. 2008; 54(9): 1580-1.
Ray Sunetra, Paulmurugan Ramasamy, Patel Manish R, Ahn Byeong C, Wu Lily, Carey Michael, Gambhir Sanjiv S Noninvasive imaging of therapeutic gene expression using a bidirectional transcriptional amplification strategy. Molecular therapy : the journal of the American Society of Gene Therapy. 2008; 16(11): 1848-56.
Ray, S. Paulmurugan, R. Patel, M. R. Ahn, B. C. Wu, L. Carey, M. Gambhir, S. S. Noninvasive imaging of therapeutic gene expression using a bidirectional transcriptional amplification strategy. Mol Ther. 2008; 16(11): 1848-56.
Burton Jeremy B, Priceman Saul J, Sung James L, Brakenhielm Ebba, An Dong Sung, Pytowski Bronislaw, Alitalo Kari, Wu Lily Suppression of prostate cancer nodal and systemic metastasis by blockade of the lymphangiogenic axis. Cancer research. 2008; 68(19): 7828-37.
Burton, J. B. Priceman, S. J. Sung, J. L. Brakenhielm, E. An, D. S. Pytowski, B. Alitalo, K. Wu, L. Suppression of prostate cancer nodal and systemic metastasis by blockade of the lymphangiogenic axis. Cancer Research. 2008; 68(19): 7828-7837.
Figueireido M, Kao C, Wu L Advances in preclinical investigation of prostate cancer gene therapy. Mol Ther . 2007; 15(6): 1053-1064.
Figueiredo Marxa L, Kao Chinghai, Wu Lily Advances in preclinical investigation of prostate cancer gene therapy. Molecular therapy : the journal of the American Society of Gene Therapy. 2007; 15(6): 1053-64.
Brakenhielm E, Burton JB, Johnson M, Chavarria N, Morizono K, Chen I, Alitalo K, Wu L. Modulating metastasis by a lymphangiogenic switch in prostate cancer. International Journal of Cancer. 2007; 121(10): 2153-2161.
Iyer Meera, Salazar Felix B, Wu Lily, Carey Michael, Gambhir Sanjiv S Bioluminescence imaging of systemic tumor targeting using a prostate-specific lentiviral vector. Human gene therapy. 2006; 17(1): 125-32.
Iyer M, Salazar FB, Wu L, Carey M, Gambhir SS Bioluminescence imaging of systemic tumor targeting using a prostate-specific lentiviral vector. Hum Gene Ther. 2006; 17(1): 125-32.
Johnson M, Huyn S, Burton JB, Sato M, Wu L Differential biodistribution of adenoviral vector in vivo as monitored by bioluminescence imaging and quantitative polymerase chain reaction. Human Gene Therapy. 2006; 17(12): 1262-9.
Wang Yanling, Iyer Meera, Annala Alexander, Wu Lily, Carey Michael, Gambhir Sanjiv S Noninvasive indirect imaging of vascular endothelial growth factor gene expression using bioluminescence imaging in living transgenic mice. Physiological genomics. 2006; 24(2): 173-80.
Wang Y, Iyer M, Annala A, Wu L, Carey M, Gambhir SS Noninvasive indirect imaging of vascular endothelial growth factor gene expression using bioluminescence imaging in living transgenic mice. Physiol Genomics. 2006; 24(2): 173-80.
Figueiredo Marxa L, Sato Makoto, Johnson Mai, Wu Lily Specific targeting of gene therapy to prostate cancer using a two-step transcriptional amplification system. Future oncology (London, England). 2006; 2(3): 391-406.
Iyer, M. Sato, M. Johnson, M. Gambhir, S. S. Wu, L. Applications of molecular imaging in cancer gene therapy. Curr Gene Ther. 2005; 5(6): 607-18.
Iyer Meera, Sato Makoto, Johnson Mai, Gambhir Sanjiv S, Wu Lily Applications of molecular imaging in cancer gene therapy. Current gene therapy. 2005; 5(6): 607-18.
Sato M, Johnson M, Zhang L, Gambhir SS, Carey M, Wu L Functionality of androgen receptor-based gene expression imaging in hormone refractory prostate cancer. Clinical cancer research : an official journal of the American Association for Cancer Research. 2005; 11(10): 3743-9.
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Sato Makoto, Johnson Mai, Zhang Liqun, Gambhir Sanjiv S, Carey Michael, Wu Lily Functionality of androgen receptor-based gene expression imaging in hormone refractory prostate cancer. Clinical cancer research : an official journal of the American Association for Cancer Research. 2005; 11(10): 3743-9.
Ilagan Romyla, Zhang Liquin Joann, Pottratz Jill, Le Kim, Salas Sussan, Iyer Meera, Wu Lily, Gambhir Sanjiv S, Carey Michael Imaging androgen receptor function during flutamide treatment in the LAPC9 xenograft model. Molecular cancer therapeutics. 2005; 4(11): 1662-9.
Ilagan R, Zhang L, Pottratz J, Le K, Salas S, Iyer M, Wu L, Gambhir SS, Carey M Imaging androgen receptor function during flutamide treatment in the LAPC9 xenograft model. Mol Can Ther. 2005; 4(11): 1662-9.
Morizono Kouki, Xie Yiming, Ringpis Gene-Errol, Johnson Mai, Nassanian Hoorig, Lee Benhur, Wu Lily, Chen Irvin S Y Lentiviral vector retargeting to P-glycoprotein on metastatic melanoma through intravenous injection. Nature medicine. 2005; 11(3): 346-52.
Morizono K, Xie Y, Ringpis G, Johnson M, Nassanian H, Lee B, Wu L, Chen ISY Lentiviral vector retargeting to P-glycoprotein on metastatic melanoma through intravenous injection. Nature Medicine. 2005; 11(3): 346-352.
Johnson Mai, Sato Makoto, Burton Jeremy, Gambhir Sanjiv S, Carey Michael, Wu Lily Micro-PET/CT monitoring of herpes thymidine kinase suicide gene therapy in a prostate cancer xenograft: the advantage of a cell-specific transcriptional targeting approach. Molecular imaging. 2005; 4(4): 463-72.
Johnson M, Sato M, Burton J, Gambhir SS, Carey M, Wu L MicroPET/CT monitoring of herpes thymidine kinase suicide gene therapy in a prostate cancer xenograft: the advantage of a cell-specific transcriptional targeting approach. Molecular Imaging 2005; (4): 463-472.
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Iyer M, Salazar FB, Lewis X, Zhang L, Wu L, Carey M, Gambhir SS Non-invasive imaging of a transgenic mouse model using a prostate-specific two-step transcriptional amplification strategy. Transgenic Res. 2005; 14(1): 47-55.
Björndahl Meit A, Cao Renhai, Burton Jeremy B, Brakenhielm Ebba, Religa Piotr, Galter Dagmar, Wu Lily, Cao Yihai Vascular endothelial growth factor-a promotes peritumoral lymphangiogenesis and lymphatic metastasis. Cancer research. 2005; 65(20): 9261-8.
Bjorndahl MA, Cao R, Burton JB, Brakenhielm E, Religa P, Galter D, Wu L, Cao Y Vascular endothelial growth factor-a promotes peritumoral lymphangiogenesis and lymphatic metastasis. Can Res. 2005; 65(20): 9261-8.
Iyer Meera, Salazar Felix B, Lewis Xiaoman, Zhang Liqun, Carey Michael, Wu Lily, Gambhir Sanjiv S Noninvasive imaging of enhanced prostate-specific gene expression using a two-step transcriptional amplification-based lentivirus vector. Molecular therapy : the journal of the American Society of Gene Therapy. 2004; 10(3): 545-52.
Iyer M, Salazar FB, Lewis X, Zhang L, Carey M, Wu L, Gambhir SS Noninvasive imaging of enhanced prostate-specific gene expression using a two-step transcriptional amplification-based lentivirus vector. Molecular therapy : the journal of the American Society of Gene Therapy. 2004; 10(3): 545-52.
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Ray, S. Paulmurugan, R. Hildebrandt, I. Iyer, M. Wu, L. Carey, M. Gambhir, S. S. Novel bidirectional vector strategy for amplification of therapeutic and reporter gene expression. Hum Gene Ther. 2004; 15(7): 681-90.
Ray Sunetra, Paulmurugan Ramasamy, Hildebrandt Isabel, Iyer Meera, Wu Lily, Carey Michael, Gambhir Sanjiv S Novel bidirectional vector strategy for amplification of therapeutic and reporter gene expression. Human gene therapy. 2004; 15(7): 681-90.
Wu Lily, Sato Makoto Integrated, molecular engineering approaches to develop prostate cancer gene therapy. Current gene therapy. 2003; 3(5): 452-67.
Wu L, Sato M Integrated, molecular engineering approaches to develop prostate cancer gene therapy. Current gene therapy. 2003; 3(5): 452-67.
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Zhang Liqun, Johnson Mai, Le Kim H, Sato Makoto, Ilagan Romyla, Iyer Meera, Gambhir Sanjiv S, Wu Lily, Carey Michael Interrogating androgen receptor function in recurrent prostate cancer. Cancer research. 2003; 63(15): 4552-60.
Zhang L, Johnson M, Le KH, Sato M, Ilagan R, Iyer M, Gambhir SS, Wu L, Carey M Interrogating androgen receptor function in recurrent prostate cancer. Cancer research. . 2003; 63(15): 4552-60.
Sato Makoto, Johnson Mai, Zhang Liqun, Zhang Baohui, Le Kim, Gambhir Sanjiv S, Carey Michael, Wu Lily Optimization of adenoviral vectors to direct highly amplified prostate-specific expression for imaging and gene therapy. Molecular therapy : the journal of the American Society of Gene Therapy. 2003; 8(5): 726-37.
Sato M, Johnson M, Zhang L, Zhang B, Le K, Gambhir SS, Carey M, Wu L Optimization of adenoviral vectors to direct highly amplified prostate-specific expression for imaging and gene therapy. Molecular therapy : the journal of the American Society of Gene Therapy. 2003; 8(5): 726-37.
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Inubushi Masayuki, Wu Joseph C, Gambhir Sanjiv S, Sundaresan Gobalakrishnan, Satyamurthy Nagichettiar, Namavari Mohammad, Yee Simon, Barrio Jorge R, Stout David, Chatziioannou Arion F, Wu Lily, Schelbert Heinrich R Positron-emission tomography reporter gene expression imaging in rat myocardium. Circulation. 2003; 107(2): 326-32.
Wu L, Johnson M, Sato M Transcriptionally targeted gene therapy to detect and treat cancer. Trends in molecular medicine. 2003; 9(10): 421-9.
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Wu Lily, Johnson Mai, Sato Makoto Transcriptionally targeted gene therapy to detect and treat cancer. Trends in molecular medicine. 2003; 9(10): 421-9.
Zhang Liqun, Adams Jason Yeates, Billick Erika, Ilagan Romyla, Iyer Meera, Le Kim, Smallwood Andrea, Gambhir Sanjiv S, Carey Michael, Wu Lily Molecular engineering of a two-step transcription amplification (TSTA) system for transgene delivery in prostate cancer. Molecular therapy : the journal of the American Society of Gene Therapy. 2002; 5(3): 223-32.
Zhang L, Adams JY, Billick E, Ilagan R, Iyer M, Le K, Smallwood A, Gambhir SS, Carey M, Wu L Molecular engineering of a two-step transcription amplification (TSTA) system for transgene delivery in prostate cancer. Molecular therapy : the journal of the American Society of Gene Therapy. . 2002; 5(3): 223-32.
Pantuck Allan J, Matherly Jamie, Zisman Amnon, Nguyen David, Berger Frank, Gambhir Sanjiv S, Black Margaret E, Belldegrun Arie, Wu Lily Optimizing prostate cancer suicide gene therapy using herpes simplex virus thymidine kinase active site variants. Human gene therapy. 2002; 13(7): 777-89.
Pantuck AJ, Matherly J, Zisman A, Nguyen D, Berger F, Gambhir SS, Black ME, Belldegrun A, Wu L Optimizing prostate cancer suicide gene therapy using herpes simplex virus thymidine kinase active site variants. Human gene therapy. . 2002; 13(7): 777-89.
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Adams JY, Sato M, Johnson M, Berger F, Gambhir S S, Carey M, Iruela-Arispe ML, Wu L. Visualization of advanced human prostate cancer lesions in living mice by a targeted gene transfer vector and optical imaging. Nature Medicine 2002; August, 8, 8: 891-896.
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Adams Jason Y, Johnson Mai, Sato Makoto, Berger Frank, Gambhir Sanjiv S, Carey Michael, Iruela-Arispe M Luisa, Wu Lily Visualization of advanced human prostate cancer lesions in living mice by a targeted gene transfer vector and optical imaging. Nature medicine. 2002; 8(8): 891-7.
Yaghoubi, S. S. Wu, L. Liang, Q. Toyokuni, T. Barrio, J. R. Namavari, M. Satyamurthy, N. Phelps, M. E. Herschman, H. R. Gambhir, S. S. Direct correlation between positron emission tomographic images of two reporter genes delivered by two distinct adenoviral vectors. Gene Ther. 2001; 8(14): 1072-80.
Iyer, M. Wu, L. Carey, M. Wang, Y. Smallwood, A. Gambhir, S. S. Two-step transcriptional amplification as a method for imaging reporter gene expression using weak promoters. Proc Natl Acad Sci U S A. 2001; 98(25): 14595-600.