FACULTY RESEARCH

Faculty List

Owen N. Witte, M.D.

Work Email Address: owenwitte@mednet.ucla.edu

Work Phone Number: (310) 206-0386

Laboratory Address:
MRL
Los Angeles, CA 90095

Office Address:
5-720 MRL
Los Angeles, CA 90095

Work Address:
5-748 MRL
CAMPUS - 166222
CA 90095

Department / Division Affiliations
Director, Eli & Edythe Broad Center of Regenerative Medicine & Stem Cell Research
President's Chair in Developmental Immunology, Microbiology, Immunology & Molecular Genetics
Distinguished Professor, Microbiology, Immunology & Molecular Genetics
Co-Leader, Pilot and Collaborative Translational Clinical Studies Program
Professor, Molecular & Medical Pharmacology
Member, Internal Advisory Board, CTSI, California NanoSystems Institute, Center for Duchenne Muscular Dystrophy, Immunity, Microbes, & Molecular Pathology GPB Home Area, JCCC Genitourinary Oncology Program Area, JCCC Tumor Immunology Program Area, Molecular Pharmacology GPB Home Area
Faculty, Molecular Biology IDP
Investigator, Howard Hughes Medical Institute

Research Interests
We are interested in the development of the immune response, growth regulation mechanisms used in leukemias and during metastasis of certain epithelial cancers to the bone marrow, and the application of new quantitative processes of whole-animal imaging. We have used positron emission tomography (PET) reporter gene strategies to mark populations of lymphocytes reactive to antigens on solid tumors and follow lymphocyte homing and expansion in living mice. Many of these techniques may be suitable for evaluation of human anti-tumor responses. Our laboratory has contributed major findings in the following areas: (i) Therapeutic Strategies for Ph Chromosome?Positive Leukemias. Human chronic myelogenous leukemia (CML) is associated with the Philadelphia chromosome, which produces the BCR-ABL tyrosine kinase oncogene. Our laboratory demonstrated that this kinase is critical for the leukemic phenotype of CML and related types of leukemia. Targeted drug therapy for CML (Gleevec and other drugs) that inhibits the enzymatic activity of BCR-ABL has been very successful for treatment of the chronic phase of CML but has been less effective for the blast crisis stage of CML or the initial treatment of Ph-positive ALL. In both of these aggressive leukemias, additional genetic changes complement the action of BCR-ABL and the malignancy continues to grow in the face of the kinase-targeted therapy. Although some of this lack of responsiveness is due to mutations in the kinase that render the drug less effective, in other cases the combination of genetic changes allows the bypass of cell killing by the drug. (ii) Lymphoid Development and Immune Monitoring by Positron Emission Tomography. A family of PET probes based on FAC has been developed, and these can be used to predict the uptake of closely related chemotherapy agents; this may aid in selecting specific patients for more-personalized chemotherapy regimens. Early-phase clinical testing of the FAC family probes has been initiated, in collaboration with Caius Radu and Johannes Czernin (UCLA). (iii) Prostate Cancer Pathogenesis. To develop a system where the prostate gland could be studied analogously to blood, we adapted a previously described tissue recombination model to reconstruct prostatic tissue from dissociated single cells when combined with an inductive stromal source and transplanted into immune-deficient mice. This system allows for the purification of cells that generate prostatic structures, and also provides a way to define specific oncogenic insults or combinations capable of transforming naÔve prostate epithelium. Building on our expertise in the mouse system, we asked if we could purify distinct subsets of cells from the human prostate, using surface markers CD49f and Trop2 to define basal and luminal cells isolated from primary benign human prostate tissue. Basal cells, defined as CD49fhiTrop2+, could self-renew in vitro and generate prostatic ducts in vivo. Introduction of three oncogenes?AKT, ERG, and AR?in basal cells, but not luminal cells, was sufficient to initiate prostate adenocarcinoma. The resulting cancer had a luminal phenotype, demonstrating that a stem-like basal cell can serve as a cell of origin and differentiate into a malignant cell with a mature marker profile.


Publications
Giannoni Francesca, Hardee Cinnamon L, Wherley Jennifer, Gschweng Eric, Senadheera Shantha, Kaufman Michael L, Chan Rebecca, Bahner Ingrid, Gersuk Vivian, Wang Xiaoyan, Gjertson David, Baltimore David, Witte Owen N, Economou James S, Ribas Antoni, Kohn Donald B Allelic Exclusion and Peripheral Reconstitution by TCR Transgenic T Cells Arising From Transduced Human Hematopoietic Stem/Progenitor Cells. Molecular therapy : the journal of the American Society of Gene Therapy. 2013; 21(5): 1044-54.
Gil Jose S, Machado Hidevaldo B, Campbell Dean O, McCracken Melissa, Radu Caius, Witte Owen N, Herschman Harvey R Application of a Rapid, Simple, and Accurate Adenovirus-Based Method to Compare PET Reporter Gene/PET Reporter Probe Systems. Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging. 2013; 15(3): 273-81.
McCracken Melissa N, Gschweng Eric H, Nair-Gill Evan, McLaughlin Jami, Cooper Aaron R, Riedinger Mireille, Cheng Donghui, Nosala Christopher, Kohn Donald B, Witte Owen N Long-term in vivo monitoring of mouse and human hematopoietic stem cell engraftment with a human positron emission tomography reporter gene. Proceedings of the National Academy of Sciences of the United States of America. 2013; 110(5): 1857-62.
Ma Chao, Cheung Ann F, Chodon Thinle, Koya Richard C, Wu Zhongqi, Ng Charles, Avramis Earl, Cochran Alistair J, Witte Owen N, Baltimore David, Chmielowski Bartosz, Economou James S, Comin-Anduix Begonya, Ribas Antoni, Heath James R Multifunctional T-cell analyses to study response and progression in adoptive cell transfer immunotherapy. Cancer discovery. 2013; 3(4): 418-29.
Goldstein Andrew S, Witte Owen N A plethora of progenitors in the post-natal prostate. EMBO reports. 2012; 13(12): 1036-7.
Cai Houjian, Memarzadeh Sanaz, Stoyanova Tanya, Beharry Zanna, Kraft Andrew S, Witte Owen N Collaboration of Kras and androgen receptor signaling stimulates EZH2 expression and tumor-propagating cells in prostate cancer. Cancer research. 2012; 72(18): 4672-81.
Tsukada Satoshi, Saffran Douglas C, Rawlings David J, Parolini Ornella, Allen R Cutler, Klisak Ivana, Sparkes Robert S, Kubagawa Hiromi, Mohandas Thuluvancheri, Quan Shirley, Belmont John W, Cooper Max D, Conley Mary Ellen, Witte Owen N Deficient expression of a B cell cytoplasmic tyrosine kinase in human X-linked agammaglobulinemia. 1993. Journal of immunology (Baltimore, Md. : 1950). 2012; 188(7): 2936-47.
Janzen Deanna M, Cheng Donghui, Schafenacker Amanda M, Paik Daniel Y, Goldstein Andrew S, Witte Owen N, Jaroszewicz Artur, Pellegrini Matteo, Memarzadeh Sanaz Estrogen and progesterone together expand murine endometrial epithelial progenitor cells. Stem cells (Dayton, Ohio). 2012; 31(4): 808-22.
Jiao Jing, Hindoyan Antreas, Wang Shunyou, Tran Linh M, Goldstein Andrew S, Lawson Devon, Chen Donghui, Li Yunfeng, Guo Changyong, Zhang Baohui, Fazli Ladan, Gleave Martin, Witte Owen N, Garraway Isla P, Wu Hong Identification of CD166 as a surface marker for enriching prostate stem/progenitor and cancer initiating cells. PloS one. 2012; 7(8): e42564.
Braas Daniel, Ahler Ethan, Tam Brenna, Nathanson David, Riedinger Mirielle, Benz Matthias R, Smith Kathleen B, Eilber Fritz C, Witte Owen N, Tap William D, Wu Hong, Christofk Heather R Metabolomics strategy reveals subpopulation of liposarcomas sensitive to gemcitabine treatment. Cancer discovery. 2012; 2(12): 1109-17.
Austin Wayne R, Armijo Amanda L, Campbell Dean O, Singh Arun S, Hsieh Terry, Nathanson David, Herschman Harvey R, Phelps Michael E, Witte Owen N, Czernin Johannes, Radu Caius G Nucleoside salvage pathway kinases regulate hematopoiesis by linking nucleotide metabolism with replication stress. The Journal of experimental medicine. 2012; 209(12): 2215-28.
Drake Justin M, Graham Nicholas A, Stoyanova Tanya, Sedghi Amir, Goldstein Andrew S, Cai Houjian, Smith Daniel A, Zhang Hong, Komisopoulou Evangelia, Huang Jiaoti, Graeber Thomas G, Witte Owen N Oncogene-specific activation of tyrosine kinase networks during prostate cancer progression. Proceedings of the National Academy of Sciences of the United States of America. 2012; 109(5): 1643-1648.
Stoyanova Tanya, Goldstein Andrew S, Cai Houjian, Drake Justin M, Huang Jiaoti, Witte Owen N Regulated proteolysis of Trop2 drives epithelial hyperplasia and stem cell self-renewal via ő≤-catenin signaling. Genes & development. 2012; 26(20): 2271-85.
Paik Daniel Y, Janzen Deanna M, Schafenacker Amanda M, Velasco Victor S, Shung May S, Cheng Donghui, Huang Jiaoti, Witte Owen N, Memarzadeh Sanaz Stem-like epithelial cells are concentrated in the distal end of the fallopian tube: a site for injury and serous cancer initiation. Stem cells (Dayton, Ohio). 2012; 30(11): 2487-97.
Zong Yang, Huang Jiaoti, Sankarasharma Devipriya, Morikawa Teppei, Fukayama Masashi, Epstein Jonathan I, Chada Kiran K, Witte Owen N Stromal epigenetic dysregulation is sufficient to initiate mouse prostate cancer via paracrine Wnt signaling. Proceedings of the National Academy of Sciences of the United States of America. 2012; 109(50): E3395-404.
Goldstein Andrew S, Zong Yang, Witte Owen N A two-step toward personalized therapies for prostate cancer. Science translational medicine. 2011; 3(72): 72ps7.
Chen Aishe, Dong Lixue, Leffler Nancy R, Asch Adam S, Witte Owen N, Yang Li V Activation of GPR4 by acidosis increases endothelial cell adhesion through the cAMP/Epac pathway. PloS one. 2011; 6(11): e27586.
Tsutsui Hideaki, Valamehr Bahram, Hindoyan Antreas, Qiao Rong, Ding Xianting, Guo Shuling, Witte Owen N, Liu Xin, Ho Chih-Ming, Wu Hong An optimized small molecule inhibitor cocktail supports long-term maintenance of human embryonic stem cells. Nature communications. 2011; 2(3): 167.
Cai Houjian, Smith Daniel A, Memarzadeh Sanaz, Lowell Clifford A, Cooper Jonathan A, Witte Owen N Differential transformation capacity of Src family kinases during the initiation of prostate cancer. Proceedings of the National Academy of Sciences of the United States of America. 2011; 108(16): 6579-84.
Schwarzenberg Johannes, Radu Caius G, Benz Matthias, Fueger Barbara, Tran Andrew Q, Phelps Michael E, Witte Owen N, Satyamurthy Nagichettiar, Czernin Johannes, Schiepers Christiaan Human biodistribution and radiation dosimetry of novel PET probes targeting the deoxyribonucleoside salvage pathway. European journal of nuclear medicine and molecular imaging. 2011; 38(4): 711-21.
Cai Houjian, Babic Ivan, Wei Xiao, Huang Jiaoti, Witte Owen N Invasive prostate carcinoma driven by c-Src and androgen receptor synergy. Cancer research. 2011; 71(3): 862-72.
Wu Ting-Hsiang, Teslaa Tara, Kalim Sheraz, French Christopher T, Moghadam Shahriar, Wall Randolph, Miller Jeffery F, Witte Owen N, Teitell Michael A, Chiou Pei-Yu Photothermal nanoblade for large cargo delivery into mammalian cells. Analytical chemistry. 2011; 83(4): 1321-7.
Goldstein Andrew S, Drake Justin M, Burnes Daina L, Finley David S, Zhang Hong, Reiter Robert E, Huang Jiaoti, Witte Owen N Purification and direct transformation of epithelial progenitor cells from primary human prostate. Nature protocols. 2011; 6(5): 656-67.
Huang Xinqiang, Gschweng Eric, Van Handel Ben, Cheng Donghui, Mikkola Hanna K A, Witte Owen N Regulated expression of microRNAs-126/126* inhibits erythropoiesis from human embryonic stem cells. Blood. 2011; 117(7): 2157-65.
Memarzadeh Sanaz, Cai Houjian, Janzen Deanna M, Xin Li, Lukacs Rita, Riedinger Mireille, Zong Yang, DeGendt Karel, Verhoeven Guido, Huang Jiaoti, Witte Owen N Role of autonomous androgen receptor signaling in prostate cancer initiation is dichotomous and depends on the oncogenic signal. Proceedings of the National Academy of Sciences of the United States of America. 2011; 108(19): 7962-7.
Huang Jiaoti, Witte Owen N A seminal finding for prostate cancer?. The New England journal of medicine. 2010; 362(2): 175-6.
Lawson Devon A, Zong Yang, Memarzadeh Sanaz, Xin Li, Huang Jiaoti, Witte Owen N Basal epithelial stem cells are efficient targets for prostate cancer initiation. Proceedings of the National Academy of Sciences of the United States of America. 2010; 107(6): 2610-5.
Lukacs Rita U, Memarzadeh Sanaz, Wu Hong, Witte Owen N Bmi-1 is a crucial regulator of prostate stem cell self-renewal and malignant transformation. Cell stem cell. 2010; 7(6): 682-93.
Memarzadeh Sanaz, Zong Yang, Janzen Deanna M, Goldstein Andrew S, Cheng Donghui, Kurita Takeshi, Schafenacker Amanda M, Huang Jiaoti, Witte Owen N Cell-autonomous activation of the PI3-kinase pathway initiates endometrial cancer from adult uterine epithelium. Proceedings of the National Academy of Sciences of the United States of America. 2010; 107(40): 17298-303.
Brewer Sarah, Nair-Gill Evan, Wei Bo, Chen Ling, Li Xiaoxiao, Riedinger Mireille, Campbell Dean O, Wiltzius Stephanie, Satyamurthy Nagichettiar, Phelps Michael E, Radu Caius, Witte Owen N, Braun Jonathan Epithelial uptake of [18F]1-(2'-deoxy-2'-arabinofuranosyl) cytosine indicates intestinal inflammation in mice. Gastroenterology. 2010; 138(4): 1266-75.
Garraway Isla P, Sun Wenyi, Tran Chau P, Perner Sven, Zhang Bao, Goldstein Andrew S, Hahm Scott A, Haider Maahum, Head Christian S, Reiter Robert E, Rubin Mark A, Witte Owen N Human prostate sphere-forming cells represent a subset of basal epithelial cells capable of glandular regeneration in vivo. The Prostate. 2010; 70(5): 491-501.
Goldstein Andrew S, Huang Jiaoti, Guo Changyong, Garraway Isla P, Witte Owen N Identification of a cell of origin for human prostate cancer. Science (New York, N.Y.). 2010; 329(5991): 568-71.
Signer Robert A J, Montecino-Rodriguez Encarnacion, Witte Owen N, Dorshkind Kenneth Immature B-cell progenitors survive oncogenic stress and efficiently initiate Ph+ B-acute lymphoblastic leukemia. Blood. 2010; 116(14): 2522-30.
Lukacs Rita U, Goldstein Andrew S, Lawson Devon A, Cheng Donghui, Witte Owen N Isolation, cultivation and characterization of adult murine prostate stem cells. Nature protocols. 2010; 5(4): 702-13.
Koya Richard C, Mok Stephen, Comin-Anduix Bego√Īa, Chodon Thinle, Radu Caius G, Nishimura Michael I, Witte Owen N, Ribas Antoni Kinetic phases of distribution and tumor targeting by T cell receptor engineered lymphocytes inducing robust antitumor responses. Proceedings of the National Academy of Sciences of the United States of America. 2010; 107(32): 14286-91.
Kamei Ken-ichiro, Ohashi Minori, Gschweng Eric, Ho Quinn, Suh Jane, Tang Jinghua, For Yu Zeta Tak, Clark Amander T, Pyle April D, Teitell Michael A, Lee Ki-Bum, Witte Owen N, Tseng Hsian-Rong Microfluidic image cytometry for quantitative single-cell profiling of human pluripotent stem cells in chemically defined conditions. Lab on a chip. 2010; 10(9): 1113-9.
Shu Chengyi J, Campbell Dean O, Lee Jason T, Tran Andrew Q, Wengrod Jordan C, Witte Owen N, Phelps Michael E, Satyamurthy Nagichettiar, Czernin Johannes, Radu Caius G Novel PET probes specific for deoxycytidine kinase. Journal of nuclear medicine : official publication, Society of Nuclear Medicine. 2010; 51(7): 1092-8.
Baltimore David, Witte Owen N, Yang Lili, Economou James, Ribas Antoni Overcoming barriers to programming a therapeutic cellular immune response to fight melanoma. Pigment cell & melanoma research. 2010; 23(2): 288-9.
Nair-Gill Evan, Wiltzius Stephanie M, Wei Xiao X, Cheng Donghui, Riedinger Mireille, Radu Caius G, Witte Owen N PET probes for distinct metabolic pathways have different cell specificities during immune responses in mice. The Journal of clinical investigation. 2010; 120(6): 2005-15.
Goldstein Andrew S, Stoyanova Tanya, Witte Owen N Primitive origins of prostate cancer: in vivo evidence for prostate-regenerating cells and prostate cancer-initiating cells. Molecular oncology. 2010; 4(5): 385-96.
Toy Gerald, Austin Wayne R, Liao Hsiang-I, Cheng Donghui, Singh Arun, Campbell Dean O, Ishikawa Tomo-o, Lehmann Lynn W, Satyamurthy Nagichettiar, Phelps Michael E, Herschman Harvey R, Czernin Johannes, Witte Owen N, Radu Caius G Requirement for deoxycytidine kinase in T and B lymphocyte development. Proceedings of the National Academy of Sciences of the United States of America. 2010; 107(12): 5551-6.
Mikkola Hanna K A, Radu Caius G, Witte Owen N Targeting leukemia stem cells. Nature biotechnology. 2010; 28(3): 237-8.
Laing Rachel E, Nair-Gill Evan, Witte Owen N, Radu Caius G Visualizing cancer and immune cell function with metabolic positron emission tomography. Current opinion in genetics & development. 2010; 20(1): 100-5.
Kamei Ken-Ichiro, Guo Shuling, Yu Zeta Tak For, Takahashi Hiroko, Gschweng Eric, Suh Carol, Wang Xiaopu, Tang Jinghua, McLaughlin Jami, Witte Owen N, Lee Ki-Bum, Tseng Hsian-Rong An integrated microfluidic culture device for quantitative analysis of human embryonic stem cells. Lab on a chip. 2009; 9(4): 555-63.
Zong Yang, Xin Li, Goldstein Andrew S, Lawson Devon A, Teitell Michael A, Witte Owen N ETS family transcription factors collaborate with alternative signaling pathways to induce carcinoma from adult murine prostate cells. Proceedings of the National Academy of Sciences of the United States of America. 2009; 106(30): 12465-70.
Kottyan Leah C, Collier Ann R, Cao Khanh H, Niese Kathryn A, Hedgebeth Megan, Radu Caius G, Witte Owen N, Khurana Hershey Gurjit K, Rothenberg Marc E, Zimmermann Nives Eosinophil viability is increased by acidic pH in a cAMP- and GPR65-dependent manner. Blood. 2009; 114(13): 2774-82.
Yu Zeta Tak For, Kamei Ken-ichiro, Takahashi Hiroko, Shu Chengyi Jenny, Wang Xiaopu, He George Wenfu, Silverman Robert, Radu Caius G, Witte Owen N, Lee Ki-Bum, Tseng Hsian-Rong Integrated microfluidic devices for combinatorial cell-based assays. Biomedical microdevices. 2009; 11(3): 547-55.
Mulholland David J, Xin Li, Morim Ashkan, Lawson Devon, Witte Owen, Wu Hong Lin-Sca-1+CD49fhigh stem/progenitors are tumor-initiating cells in the Pten-null prostate cancer model. Cancer research. 2009; 69(22): 8555-62.
Kwong Gabriel A, Radu Caius G, Hwang Kiwook, Shu Chengyi J, Ma Chao, Koya Richard C, Comin-Anduix Begonya, Hadrup Sine Reker, Bailey Ryan C, Witte Owen N, Schumacher Ton N, Ribas Antoni, Heath James R Modular nucleic acid assembled p/MHC microarrays for multiplexed sorting of antigen-specific T cells. Journal of the American Chemical Society. 2009; 131(28): 9695-703.
Laing Rachel E, Walter Martin A, Campbell Dean O, Herschman Harvey R, Satyamurthy Nagichettiar, Phelps Michael E, Czernin Johannes, Witte Owen N, Radu Caius G Noninvasive prediction of tumor responses to gemcitabine using positron emission tomography. Proceedings of the National Academy of Sciences of the United States of America. 2009; 106(8): 2847-52.
Shu Chengyi J, Radu Caius G, Shelly Stephanie M, Vo Dan D, Prins Robert, Ribas Antoni, Phelps Michael E, Witte Owen N Quantitative PET reporter gene imaging of CD8+ T cells specific for a melanoma-expressed self-antigen. International immunology. 2009; 21(2): 155-65.
Signer Robert A J, Montecino-Rodriguez Encarnacion, Witte Owen N, Dorshkind Kenneth Aging and cancer resistance in lymphoid progenitors are linked processes conferred by p16Ink4a and Arf. Genes & development. 2008; 22(22): 3115-20.
Prins Robert M, Shu Chengyi J, Radu Caius G, Vo Dan D, Khan-Farooqi Haumith, Soto Horacio, Yang Meng-Yin, Lin Muh-Shi, Shelly Stephanie, Witte Owen N, Ribas Antoni, Liau Linda M Anti-tumor activity and trafficking of self, tumor-specific T cells against tumors located in the brain. Cancer immunology, immunotherapy : CII. 2008; 57(9): 1279-89.
Moore Miranda L, Teitell Michael A, Kim Yoon, Watabe Tetsuro, Reiter Robert E, Witte Owen N, Dubey Purnima Deletion of PSCA increases metastasis of TRAMP-induced prostate tumors without altering primary tumor formation. The Prostate. 2008; 68(2): 139-51.
Valamehr Bahram, Jonas Steven J, Polleux Julien, Qiao Rong, Guo Shuling, Gschweng Eric H, Stiles Bangyan, Kam Korey, Luo Tzy-Jiun M, Witte Owen N, Liu Xin, Dunn Bruce, Wu Hong Hydrophobic surfaces for enhanced differentiation of embryonic stem cell-derived embryoid bodies. Proceedings of the National Academy of Sciences of the United States of America. 2008; 105(38): 14459-64.
Peter Christoph, Waibel Michaela, Radu Caius G, Yang Li V, Witte Owen N, Schulze-Osthoff Klaus, Wesselborg Sebastian, Lauber Kirsten Migration to apoptotic "find-me" signals is mediated via the phagocyte receptor G2A. The Journal of biological chemistry. 2008; 283(9): 5296-305.
Radu Caius G, Shu Chengyi J, Nair-Gill Evan, Shelly Stephanie M, Barrio Jorge R, Satyamurthy Nagichettiar, Phelps Michael E, Witte Owen N Molecular imaging of lymphoid organs and immune activation by positron emission tomography with a new [18F]-labeled 2'-deoxycytidine analog. Nature medicine. 2008; 14(7): 783-8.
Nair-Gill Evan D, Shu Chengyi J, Radu Caius G, Witte Owen N Non-invasive imaging of adaptive immunity using positron emission tomography. Immunological reviews. 2008; 221(9): 214-28.
Lo Roger S, Witte Owen N Transforming growth factor-beta activation promotes genetic context-dependent invasion of immortalized melanocytes. Cancer research. 2008; 68(11): 4248-57.
Goldstein Andrew S, Lawson Devon A, Cheng Donghui, Sun Wenyi, Garraway Isla P, Witte Owen N Trop2 identifies a subpopulation of murine and human prostate basal cells with stem cell characteristics. Proceedings of the National Academy of Sciences of the United States of America. 2008; 105(52): 20882-7.
Signer Robert A J, Montecino-Rodriguez Encarnacion, Witte Owen N, McLaughlin Jami, Dorshkind Kenneth Age-related defects in B lymphopoiesis underlie the myeloid dominance of adult leukemia. Blood. 2007; 110(6): 1831-9.
Bailey Ryan C, Kwong Gabriel A, Radu Caius G, Witte Owen N, Heath James R DNA-encoded antibody libraries: a unified platform for multiplexed cell sorting and detection of genes and proteins. Journal of the American Chemical Society. 2007; 129(7): 1959-67.
Memarzadeh Sanaz, Xin Li, Mulholland David J, Mansukhani Alka, Wu Hong, Teitell Michael A, Witte Owen N Enhanced paracrine FGF10 expression promotes formation of multifocal prostate adenocarcinoma and an increase in epithelial androgen receptor. Cancer cell. 2007; 12(6): 572-85.
Lawson Devon A, Xin Li, Lukacs Rita U, Cheng Donghui, Witte Owen N Isolation and functional characterization of murine prostate stem cells. Proceedings of the National Academy of Sciences of the United States of America. 2007; 104(1): 181-6.
Dorshkind Kenneth, Witte Owen N Linking the hematopoietic microenvironment to imatinib-resistant Ph+ B-ALL. Genes & development. 2007; 21(18): 2249-52.
Radu Caius G, Shu Chengyi J, Shelly Stephanie M, Phelps Michael E, Witte Owen N Positron emission tomography with computed tomography imaging of neuroinflammation in experimental autoimmune encephalomyelitis. Proceedings of the National Academy of Sciences of the United States of America. 2007; 104(6): 1937-42.
Xin Li, Lukacs Rita U, Lawson Devon A, Cheng Donghui, Witte Owen N Self-renewal and multilineage differentiation in vitro from murine prostate stem cells. Stem cells (Dayton, Ohio). 2007; 25(11): 2760-9.
Lawson Devon A, Witte Owen N Stem cells in prostate cancer initiation and progression. The Journal of clinical investigation. 2007; 117(8): 2044-50.
McLaughlin Jami, Cheng Donghui, Singer Oded, Lukacs Rita U, Radu Caius G, Verma Inder M, Witte Owen N Sustained suppression of Bcr-Abl-driven lymphoid leukemia by microRNA mimics. Proceedings of the National Academy of Sciences of the United States of America. 2007; 104(51): 20501-6.
Yang Li V, Radu Caius G, Roy Meenakshi, Lee Sunyoung, McLaughlin Jami, Teitell Michael A, Iruela-Arispe M Luisa, Witte Owen N Vascular abnormalities in mice deficient for the G protein-coupled receptor GPR4 that functions as a pH sensor. Molecular and cellular biology. 2007; 27(4): 1334-47.
Khan Abdul Q, Sen Goutam, Guo Shuling, Witte Owen N, Snapper Clifford M Induction of in vivo antipolysaccharide immunoglobulin responses to intact Streptococcus pneumoniae is more heavily dependent on Btk-mediated B-cell receptor signaling than antiprotein responses. Infection and immunity. 2006; 74(2): 1419-24.
Guo Shuling, Wahl Matthew I, Witte Owen N Mutational analysis of the SH2-kinase linker region of Bruton's tyrosine kinase defines alternative modes of regulation for cytoplasmic tyrosine kinase families. International immunology. 2006; 18(1): 79-87.
Lei Qunying, Jiao Jing, Xin Li, Chang Chun-Ju, Wang Shunyou, Gao Jing, Gleave Martin E, Witte Owen N, Liu Xin, Wu Hong NKX3.1 stabilizes p53, inhibits AKT activation, and blocks prostate cancer initiation caused by PTEN loss. Cancer cell. 2006; 9(5): 367-78.
Radu Caius G, Cheng Donghui, Nijagal Amar, Riedinger Mireille, McLaughlin Jami, Yang Li V, Johnson James, Witte Owen N Normal immune development and glucocorticoid-induced thymocyte apoptosis in mice deficient for the T-cell death-associated gene 8 receptor. Molecular and cellular biology. 2006; 26(2): 668-77.
Xin Li, Teitell Michael A, Lawson Devon A, Kwon Andrew, Mellinghoff Ingo K, Witte Owen N Progression of prostate cancer by synergy of AKT with genotropic and nongenotropic actions of the androgen receptor. Proceedings of the National Academy of Sciences of the United States of America. 2006; 103(20): 7789-94.
Wang Shunyou, Garcia Alejandro J, Wu Michelle, Lawson Devon A, Witte Owen N, Wu Hong Pten deletion leads to the expansion of a prostatic stem/progenitor cell subpopulation and tumor initiation. Proceedings of the National Academy of Sciences of the United States of America. 2006; 103(5): 1480-5.
Radu Caius G, Nijagal Amar, McLaughlin Jami, Wang Li, Witte Owen N Differential proton sensitivity of related G protein-coupled receptors T cell death-associated gene 8 and G2A expressed in immune cells. Proceedings of the National Academy of Sciences of the United States of America. 2005; 102(5): 1632-7.
Yang Li V, Radu Caius G, Wang Li, Riedinger Mireille, Witte Owen N Gi-independent macrophage chemotaxis to lysophosphatidylcholine via the immunoregulatory GPCR G2A. Blood. 2005; 105(3): 1127-34.
Wang Li, Radu Caius G, Yang Li V, Bentolila Laurent A, Riedinger Mireille, Witte Owen N Lysophosphatidylcholine-induced surface redistribution regulates signaling of the murine G protein-coupled receptor G2A. Molecular biology of the cell. 2005; 16(5): 2234-47.
Lee Chung-Cheng, Sui Guodong, Elizarov Arkadij, Shu Chengyi Jenny, Shin Young-Shik, Dooley Alek N, Huang Jiang, Daridon Antoine, Wyatt Paul, Stout David, Kolb Hartmuth C, Witte Owen N, Satyamurthy Nagichettiar, Heath James R, Phelps Michael E, Quake Stephen R, Tseng Hsian-Rong Multistep synthesis of a radiolabeled imaging probe using integrated microfluidics. Science (New York, N.Y.). 2005; 310(5755): 1793-6.
Witte Owen N, Kabarowski Janusz H, Xu Yan, Le Lu Q, Zhu Kui Retraction. Science (New York, N.Y.). 2005; 307(5707): 206.
Xin Li, Lawson Devon A, Witte Owen N The Sca-1 cell surface marker enriches for a prostate-regenerating cell subpopulation that can initiate prostate tumorigenesis. Proceedings of the National Academy of Sciences of the United States of America. 2005; 102(19): 6942-7.
Witte Owen UCLA director gets the goods as California makes good on its stem cell initiative. Interview by Stacie Bloom. The Journal of clinical investigation. 2005; 115(11): 2958.
Beillard Emmanuel, Witte Owen N Unraveling kinase signaling pathways with chemical genetic and chemical proteomic approaches. Cell cycle (Georgetown, Tex.). 2005; 4(3): 434-7.
Shu Chengyi J, Guo Shuling, Kim Young J, Shelly Stephanie M, Nijagal Amar, Ray Pritha, Gambhir Sanjiv S, Radu Caius G, Witte Owen N Visualization of a primary anti-tumor immune response by positron emission tomography. Proceedings of the National Academy of Sciences of the United States of America. 2005; 102(48): 17412-7.
Guo Shuling, Ferl Gregory Z, Deora Rajendar, Riedinger Mireille, Yin Sheng, Kerwin James L, Loo Joseph A, Witte Owen N A phosphorylation site in Bruton's tyrosine kinase selectively regulates B cell calcium signaling efficiency by altering phospholipase C-gamma activation. Proceedings of the National Academy of Sciences of the United States of America. 2004; 101(39): 14180-5.
Dorshkind Kenneth, Witte Owen Got MLL? Definitive hematopoiesis requires MLL gene expression. Molecular cell. 2004; 13(6): 765-6.
Kim Young J, Dubey Purnima, Ray Pritha, Gambhir Sanjiv S, Witte Owen N Multimodality imaging of lymphocytic migration using lentiviral-based transduction of a tri-fusion reporter gene. Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging. 2004; 6(5): 331-40.
Kharas Michael G, Deane Jonathan A, Wong Stephane, O'Bosky Karen R, Rosenberg Naomi, Witte Owen N, Fruman David A Phosphoinositide 3-kinase signaling is essential for ABL oncogene-mediated transformation of B-lineage cells. Blood. 2004; 103(11): 4268-75.
Yu Phyllis W, Tabuchi Ruby S, Kato Roberta M, Astrakhan Alexander, Humblet-Baron Stephanie, Kipp Kevin, Chae Keun, Ellmeier Wilfried, Witte Owen N, Rawlings David J Sustained correction of B-cell development and function in a murine model of X-linked agammaglobulinemia (XLA) using retroviral-mediated gene transfer. Blood. 2004; 104(5): 1281-90.
Radu Caius G, Yang Li V, Riedinger Mireille, Au Matthew, Witte Owen N T cell chemotaxis to lysophosphatidylcholine through the G2A receptor. Proceedings of the National Academy of Sciences of the United States of America. 2004; 101(1): 245-50.
Wong Stephane, Witte Owen N The BCR-ABL story: bench to bedside and back. Annual review of immunology. 2004; 22(1): 247-306.
Wong Stephane, McLaughlin Jami, Cheng Donghui, Witte Owen N Cell context-specific effects of the BCR-ABL oncogene monitored in hematopoietic progenitors. Blood. 2003; 101(10): 4088-97.
Ferl Gregory Z, Timmerman John M, Witte Owen N Extending the utility of gene profiling data by bridging microarray platforms. Proceedings of the National Academy of Sciences of the United States of America. 2003; 100(19): 10585-7.
Whyburn Lindsey R, Halcomb Kristina E, Contreras Cristina M, Pappu Rajita, Witte Owen N, Chan Andrew C, Satterthwaite Anne B Haploinsufficiency of B cell linker protein enhances B cell signaling defects in mice expressing a limiting dosage of Bruton's tyrosine kinase. International immunology. 2003; 15(3): 383-92.
Wong Stephane, McLaughlin Jami, Cheng Donghui, Shannon Kevin, Robb Lorraine, Witte Owen N IL-3 receptor signaling is dispensable for BCR-ABL-induced myeloproliferative disease. Proceedings of the National Academy of Sciences of the United States of America. 2003; 100(20): 11630-5.
Xin Li, Ide Hisamitsu, Kim Yoon, Dubey Purnima, Witte Owen N In vivo regeneration of murine prostate from dissociated cell populations of postnatal epithelia and urogenital sinus mesenchyme. Proceedings of the National Academy of Sciences of the United States of America. 2003; 100 Suppl 1(20): 11896-903.
Dubey Purnima, Su Helen, Adonai Nona, Du Shouying, Rosato Antonio, Braun Jonathan, Gambhir Sanjiv S, Witte Owen N Quantitative imaging of the T cell antitumor response by positron-emission tomography. Proceedings of the National Academy of Sciences of the United States of America. 2003; 100(3): 1232-7.
Whyburn Lindsey R, Halcomb Kristina E, Contreras Cristina M, Lowell Clifford A, Witte Owen N, Satterthwaite Anne B Reduced dosage of Bruton's tyrosine kinase uncouples B cell hyperresponsiveness from autoimmunity in lyn-/- mice. Journal of immunology (Baltimore, Md. : 1950). 2003; 171(4): 1850-8.
Fujimoto Manabu, Poe Jonathan C, Satterthwaite Anne B, Wahl Matthew I, Witte Owen N, Tedder Thomas F Complementary roles for CD19 and Bruton's tyrosine kinase in B lymphocyte signal transduction. Journal of immunology (Baltimore, Md. : 1950). 2002; 168(11): 5465-76.
Ide Hisamitsu, Seligson David B, Memarzadeh Sanaz, Xin Li, Horvath Steve, Dubey Purnima, Flick Maryann B, Kacinski Barry M, Palotie Aarno, Witte Owen N Expression of colony-stimulating factor 1 receptor during prostate development and prostate cancer progression. Proceedings of the National Academy of Sciences of the United States of America. 2002; 99(22): 14404-9.
Watabe Tetsuro, Lin Mark, Ide Hisamitsu, Donjacour Annemarie A, Cunha Gerald R, Witte Owen N, Reiter Robert E Growth, regeneration, and tumorigenesis of the prostate activates the PSCA promoter. Proceedings of the National Academy of Sciences of the United States of America. 2002; 99(1): 401-6.
Kabarowski Janusz H S, Xu Yan, Witte Owen N Lysophosphatidylcholine as a ligand for immunoregulation. Biochemical pharmacology. 2002; 64(2): 161-7.
Fruman David A, Ferl Gregory Z, An Sam S, Donahue Amber C, Satterthwaite Anne B, Witte Owen N Phosphoinositide 3-kinase and Bruton's tyrosine kinase regulate overlapping sets of genes in B lymphocytes. Proceedings of the National Academy of Sciences of the United States of America. 2002; 99(1): 359-64.
Le Lu Q, Kabarowski Janusz H S, Wong Stephane, Nguyen Khoi, Gambhir Sanjiv S, Witte Owen N Positron emission tomography imaging analysis of G2A as a negative modifier of lymphoid leukemogenesis initiated by the BCR-ABL oncogene. Cancer cell. 2002; 1(4): 381-91.
Wu Jinzi J, Afar Daniel E H, Phan Hoang, Witte Owen N, Lam Kit S Recognition of multiple substrate motifs by the c-ABL protein tyrosine kinase. Combinatorial chemistry & high throughput screening. 2002; 5(1): 83-91.