FACULTY RESEARCH

Faculty List

Samson A. Chow, Ph.D.

Work Email Address: schow@mednet.ucla.edu

Work Phone Number: (310) 825-9600

Chow Lab

Laboratory Address:
23-133 CHS
Los Angeles, CA 90095

Office Address:
23-133B CHS
Los Angeles, CA 90095

Department / Division Affiliations
Vice Chair, and Professor, Molecular & Medical Pharmacology
Member, Tumor Immunology Program at the Jonsson Comprehensive Cancer Center (JCCC)

Research Interests
Retroviruses are RNA viruses that replicate through a DNA intermediate. In humans, infection by retroviruses can lead to AIDS, lymphoma, and leukemia. My present studies focus on understanding the molecular mechanisms of the integration reaction catalyzed by the viral protein, integrase, purified from the human immunodeficiency virus (HIV), and examining possible chromosomal instability as a result of retroviral integration. Owing to their integration properties, retroviruses are also widely used as vectors for genetic engineering and gene replacement therapy. Progress in understanding the mechanism of integration may lead to development of still more powerful tools for molecular medicine. A related project is aimed at introducing exogenous DNA into specific target sites by use of fusion proteins consisting of HIV integrase and sequence-specific DNA binding proteins. Other research interests include feline immunodeficiency virus (FIV) and the development of FIV infection in cats as a model for HIV infection and anti-retroviral therapy in humans.

For more detailed information, please visit the lab website.



Publications

A selected list of publications:

Dobard, C.W., Briones, M. and Chow, S.A.   Molecular mechanisms by which HIV-1 integrase stimulates the early steps of reverse transcription, J Virol , 2007; 81: 10037-10046.
Dong, B., Kim, S., Hong, S., Das Gupta, J., Malathi, K., Klein, E.A., Ganem, D., DeRisi, J., Chow, S.A. and Silverman, R.H.   An infectious retrovirus susceptible to an interferon antiviral pathway from human prostate tumors, Pro Natl Acad Sci USA, 2007; 104(5): 1655-1660.
Vatakis, D.N., Bristol, G., Wilkinson, T.A., Chow, S.A. and Zack, J.A.   Immediate activation to rescue efficient HIV replication in quiescent CD4+ T cells, J Virol , 2007; 81: 3574-3582.
Kim, S. Kim, Y. Liang, T. Sinsheimer, J. S. Chow, S. A.   A high-throughput method for cloning and sequencing human immunodeficiency virus type 1 integration sites J Virol, 2006; 80(22): 11313-21.
Brooks, D. G. Cohen, M. D. Jamieson, B. D. Poon, B. Kitchen, S. G. Chow, S. A. Chen, I. S. Zack, J. A. Koka, P. S.   Rapid size dependent deletion of foreign gene sequences inserted into attenuated HIV-1 upon infection in vivo: implications for vaccine development Curr HIV Res, 2005; 3(4): 377-92.
Wilkinson, T.A, Tan, W. and Chow, S.A.   Safe delivery of therapeutic genes into specific chromosomal sites using engineered retroviral integrase, Gene Ther. Regulation, 2004; 2(4): 369-387.
Woodward CL, Wang Y, Dixon WJ, Htun H, Chow SA   Subcellular localization of feline immunodeficiency virus integrase and mapping of its karyophilic determinant J Virol , 2003; 77(8): 4516-27.
Holmes-Son ML, Chow SA   Correct integration mediated by integrase-LexA fusion proteins incorporated into HIV-1 Mol Ther, 2002; 5(4): 360-70.
Holmes-Son ML, Appa RS, Chow SA   Molecular genetics and target site specificity of retroviral integration Adv Genet , 2001; 43: 33-69.
Appa RS, Shin C-G, Lee P, Chow SA   Role of the nonspecific DNA-binding region and a-helices within the core domain of retroviral integrase in selecting target DNA sites for integration, J Biol Chem, 2001; 276: 45848-45855.
Zhu K, Cordeiro ML, Atienza J, Robinson WE, Chow SA   Irreversible inhibition of human immunodeficiency virus type 1 integrase by dicaffeoylquinic acids J Virol , 1999; 73(4): 3309-16.
Shibagaki Y, Chow SA   Central core domain of retroviral integrase is responsible for target site selection, J Biol Chem, 1997; 272: 8361-8369.
Shibagaki Y, Holmes ML, Appa RS, Chow SA   Characterization of feline immunodeficiency virus integrase and analysis of functional domains Virology , 1997; 230(1): 1-10.
Scottoline BP, Chow S, Ellison V, Brown PO   Disruption of the terminal base pairs of retroviral DNA during integration Genes Dev , 1997; 11(3): 371-82.
Chow SA   In vitro assays for activities of retroviral integrase, Methods: a Companion to Methods in Enzymology, 1997; 12: 306-317.
Robinson WE, Cordeiro M, Abdel-Malek S, Jia Q, Chow SA, Reinecke MG, Mitchell WM   Dicaffeoylquinic acid inhibitors of human immunodeficiency virus integrase: inhibition of the core catalytic domain of human immunodeficiency virus integrase Mol Pharmacol , 1996; 50(4): 846-55.
Robinson Jr. WE, Reinicke MG, Abdel-Malek S, Jia Q, Chow SA   Inhibitors of HIV-1 replication that inhibit HIV integrase, Proc Natl Acad Sci USA, 1996; 93: 6326-6331.
Chow SA, Vincent KA, Ellison V, Brown PO   Reversal of integration and DNA splicing mediated by integrase of human immunodeficiency virus, Science, 1992; 255: 723-726.
Vatakis, D. N. Kim, S. Kim, N. Chow, S. A. Zack, J. A.   Human immunodeficiency virus integration efficiency and site selection in quiescent CD4+ T cells J. Virol, 2009; 83(12): 6222-6533.
Woodward, C. L. Prakobwanakit, S. Mosessian, S. Chow, S. A.   Integrase interacts with nucleoporin NUP153 to mediate the nuclear import of human immunodeficiency virus type 1 J. Virol, 2009; 83(13): 6522-6533.
Kim, S. Kim, N. Dong, B. Boren, D. Lee, S. A. Das Gupta, J. Gaughan, C. Klein, E. A. Lee, C. Silverman, R. H. Chow, S. A.   Integration site preference of xenotropic murine leukemia virus-related virus, a new human retrovirus associated with prostate cancer, J. Virol, 2008; 82(20): 9964-9977.
Wilkinson, T. A. Januszyk, K. Phillips, M. L. Tekeste, S. S. Zhang, M. Miller, J. T. Le Grice, S. F. Clubb, R. T. Chow, S. A.   Identifying and Characterizing a Functional HIV-1 Reverse Transcriptase-binding Site on Integrase J. Biol. Chem, 2009; 284(12): 7931-7939.