FACULTY RESEARCH

Faculty List

Ting-Ting Wu, Ph.D.

Work Email Address: twu@mednet.ucla.edu

Work Phone Number: (310) 267-2218

Ting-Ting Wu Lab

Mailing Address:
10-240 Factor
650 Charles E Young Dr. South
Los Angeles, CA 90095

Department / Division Affiliations
Associate Professor, Molecular & Medical Pharmacology

Research Interests
The goal of my research is to modify herpesviruses for benefiting human health as gene delivery vectors. Specifically, in the past few years, we have conducted extensive mutagenesis on murine gammaherpesvirus-68 (MHV-68) and gained tremendous knowledge about the viral replication. We will utilize the capability of MHV-68 to infect a variety of human cells for engineering the virus as a therapeutic vehicle. The major aim is to construct a live attenuated MHV-68 virus as a vaccine vector to deliver antigens and stimulate protective immunity.


Publications
Du Yushen, Xin Li, Shi Yuan, Zhang Tian-Hao, Wu Nicholas C, Dai Lei, Gong Danyang, Brar Gurpreet, Shu Sara, Luo Jiadi, Reiley William, Tseng Yen-Wen, Bai Hongyan, Wu Ting-Ting, Wang Jieru, Shu Yuelong, Sun Ren Genome-wide identification of interferon-sensitive mutations enables influenza vaccine design. Science (New York, N.Y.). 2018; 359(6373): 290-296.
Dai Xinghong, Gong Danyang, Lim Hanyoung, Jih Jonathan, Wu Ting-Ting, Sun Ren, Zhou Z Hong Structure and mutagenesis reveal essential capsid protein interactions for KSHV replication. Nature. 2018; 553(7689): 521-525.
Du Yushen, Zhang Tian-Hao, Dai Lei, Zheng Xiaojuan, Gorin Aleksandr M, Oishi John, Wu Ting-Ting, Yoshizawa Janice M, Li Xinmin, Yang Otto O, Martinez-Maza Otoniel, Detels Roger, Sun Ren Effects of Mutations on Replicative Fitness and Major Histocompatibility Complex Class I Binding Affinity Are Among the Determinants Underlying Cytotoxic-T-Lymphocyte Escape of HIV-1 Gag Epitopes. mBio. 2017; 8(6): 290-296.
Du Yushen, Chi Xiumei, Wang Chong, Jiang Jing, Kong Fei, Yan Hongqing, Wang Xiaomei, Li Jie, Wu Nicholas C, Dai Lei, Zhang Tian-Hao, Shu Sara, Zhou Jian, Yoshizawa Janice M, Li Xinmin, Bhattacharya Debika, Wu Ting-Ting, Niu Junqi, Sun Ren Quantifying perinatal transmission of Hepatitis B viral quasispecies by tag linkage deep sequencing. Scientific reports. 2017; 7(1): 10168.
Gong Danyang, Dai Xinghong, Xiao Yuchen, Du Yushen, Chapa Travis J, Johnson Jeffrey R, Li Xinmin, Krogan Nevan J, Deng Hongyu, Wu Ting-Ting, Sun Ren Virus-Like Vesicles of Kaposi's Sarcoma-Associated Herpesvirus Activate Lytic Replication by Triggering Differentiation Signaling. Journal of virology. 2017; 91(15): 10168.
Qi Hangfei, Chu Virginia, Wu Nicholas C, Chen Zugen, Truong Shawna, Brar Gurpreet, Su Sheng-Yao, Du Yushen, Arumugaswami Vaithilingaraja, Olson C Anders, Chen Shu-Hua, Lin Chung-Yen, Wu Ting-Ting, Sun Ren Systematic identification of anti-interferon function on hepatitis C virus genome reveals p7 as an immune evasion protein. Proceedings of the National Academy of Sciences of the United States of America. 2017; 114(8): 2018-2023.
Gong Danyang, Kim Yong Hoon, Xiao Yuchen, Du Yushen, Xie Yafang, Lee Kevin K, Feng Jun, Farhat Nisar, Zhao Dawei, Shu Sara, Dai Xinghong, Chanda Sumit K, Rana Tariq M, Krogan Nevan J, Sun Ren, Wu Ting-Ting A Herpesvirus Protein Selectively Inhibits Cellular mRNA Nuclear Export. Cell host & microbe. 2016; 20(5): 642-653.
Du Yushen, Wu Nicholas C, Jiang Lin, Zhang Tianhao, Gong Danyang, Shu Sara, Wu Ting-Ting, Sun Ren Annotating Protein Functional Residues by Coupling High-Throughput Fitness Profile and Homologous-Structure Analysis. mBio. 2016; 7(6): 642-653.
Wu Nicholas C, Du Yushen, Le Shuai, Young Arthur P, Zhang Tian-Hao, Wang Yuanyuan, Zhou Jian, Yoshizawa Janice M, Dong Ling, Li Xinmin, Wu Ting-Ting, Sun Ren Coupling high-throughput genetics with phylogenetic information reveals an epistatic interaction on the influenza A virus M segment. BMC genomics. 2016; 17(6): 46.
York Autumn G, Williams Kevin J, Argus Joseph P, Zhou Quan D, Brar Gurpreet, Vergnes Laurent, Gray Elizabeth E, Zhen Anjie, Wu Nicholas C, Yamada Douglas H, Cunningham Cameron R, Tarling Elizabeth J, Wilks Moses Q, Casero David, Gray David H, Yu Amy K, Wang Eric S, Brooks David G, Sun Ren, Kitchen Scott G, Wu Ting-Ting, Reue Karen, Stetson Daniel B, Bensinger Steven J Limiting Cholesterol Biosynthetic Flux Spontaneously Engages Type I IFN Signaling. Cell. 2015; 163(7): 1716-29.
Qi Hangfei, Wu Nicholas C, Du Yushen, Wu Ting-Ting, Sun Ren High-resolution genetic profile of viral genomes: why it matters. Current opinion in virology. 2015; 14(7): 62-70.
Dai Xinghong, Gong Danyang, Xiao Yuchen, Wu Ting-Ting, Sun Ren, Zhou Z Hong CryoEM and mutagenesis reveal that the smallest capsid protein cements and stabilizes Kaposi's sarcoma-associated herpesvirus capsid. Proceedings of the National Academy of Sciences of the United States of America. 2015; 112(7): E649-56.
Al-Mawsawi Laith Q, Wu Nicholas C, Olson C Anders, Shi Vivian Cai, Qi Hangfei, Zheng Xiaojuan, Wu Ting-Ting, Sun Ren High-throughput profiling of point mutations across the HIV-1 genome. Retrovirology. 2014; 11(7): 124.
Dai Xinghong, Gong Danyang, Wu Ting-Ting, Sun Ren, Zhou Z Hong Organization of capsid-associated tegument components in Kaposi's sarcoma-associated herpesvirus. Journal of virology. 2014; 88(21): 12694-702.
Gong Danyang, Wu Nicholas C, Xie Yafang, Feng Jun, Tong Leming, Brulois Kevin F, Luan Harding, Du Yushen, Jung Jae U, Wang Cun-yu, Kang Mo Kwan, Park No-Hee, Sun Ren, Wu Ting-Ting Kaposi's sarcoma-associated herpesvirus ORF18 and ORF30 are essential for late gene expression during lytic replication. Journal of virology. 2014; 88(19): 11369-82.
Wu Nicholas C, Young Arthur P, Al-Mawsawi Laith Q, Olson C Anders, Feng Jun, Qi Hangfei, Luan Harding H, Li Xinmin, Wu Ting-Ting, Sun Ren High-throughput identification of loss-of-function mutations for anti-interferon activity in the influenza A virus NS segment. Journal of virology. 2014; 88(17): 10157-64.
Feng Jun, De Jesus Paul D, Su Victoria, Han Stephanie, Gong Danyang, Wu Nicholas C, Tian Yuan, Li Xudong, Wu Ting-Ting, Chanda Sumit K, Sun Ren RIOK3 is an adaptor protein required for IRF3-mediated antiviral type I interferon production. Journal of virology. 2014; 88(14): 7987-97.
Wei Qingshan, Qi Hangfei, Luo Wei, Tseng Derek, Ki So Jung, Wan Zhe, Göröcs Zoltán, Bentolila Laurent A, Wu Ting-Ting, Sun Ren, Ozcan Aydogan Fluorescent imaging of single nanoparticles and viruses on a smart phone. ACS nano. 2013; 7(10): 9147-55.
Wu Ting-Ting, Qian Jing, Ang Jian, Sun Ren Vaccine prospect of Kaposi sarcoma-associated herpesvirus. Current opinion in virology. 2012; 2(4): 482-8.
Wu Ting-Ting, Blackman Marcia A, Sun Ren Prospects of a novel vaccination strategy for human gamma-herpesviruses. Immunologic research. 2010; 48(1-3): 122-46.
Wu Ting-Ting, Park Tina, Kim Hana, Tran Thuy, Tong Leming, Martinez-Guzman DeeAnn, Reyes Nichole, Deng Hongyu, Sun Ren ORF30 and ORF34 are essential for expression of late genes in murine gammaherpesvirus 68. Journal of virology. 2009; 83(5): 2265-73.
Symensma Tonia L, Martinez-Guzman DeeAnn, Jia Qingmei, Bortz Eric, Wu Ting-Ting, Rudra-Ganguly Nandini, Cole Steve, Herschman Harvey, Sun Ren COX-2 induction during murine gammaherpesvirus 68 infection leads to enhancement of viral gene expression. Journal of virology. 2003; 77(23): 12753-63.